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SPOT Synthesis of Peptide Arrays on Self‐Assembled Monolayers and their Evaluation as Enzyme Substrates
Author(s) -
Laurent Nicolas,
Haddoub Rose,
Voglmeir Josef,
Wong Stephen C. C.,
Gaskell Simon J.,
Flitsch Sabine L.
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200800481
Subject(s) - monolayer , peptide , substrate (aquarium) , proteases , combinatorial chemistry , chemistry , nanotechnology , computer science , enzyme , computational biology , biochemistry , materials science , biology , ecology
Sweet SPOT : An efficient SPOT peptide synthesis methodology on self‐assembled monolayer‐coated gold surfaces was developed. Arrays of peptides and glycopeptides were subsequently used to evaluate substrate specificity of proteases and glycosyltransferases. The efficiency of all reactions could be easily monitored directly on‐chip by using MALDI‐ToF MS.