Nanomolar Heparin Detection with an Artificial Enzyme | Zendy

Vial Laurent | Zendy; Dumy Pascal | Zendy

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Nanomolar Heparin Detection with an Artificial Enzyme

Author(s) -

Vial Laurent,

Dumy Pascal

Publication year - 2008

Publication title -

chembiochem

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.05

H-Index - 126

eISSN - 1439-7633

pISSN - 1439-4227

DOI - 10.1002/cbic.200800420

Subject(s) - heparin , fluorescence , artificial enzyme , chemistry , hydrolysis , peptide , enzyme , enzymatic hydrolysis , biosensor , combinatorial chemistry , biochemistry , chromatography , physics , quantum mechanics

Detecting heparin : We report a new fluorescent approach to the detection and quantification of the biologically relevant molecule heparin. We designed and prepared a cyclic peptide that is able to catalyse the hydrolysis of a fluorogenic anionic ester (green) under low‐concentration conditions. Then we demonstrated that the inhibition of this artificial enzyme (grey) by heparin (red) allows its fluorescent sensing with a remarkable sensitivity of 13 n M .

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