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Live‐Cell Fluorescence Microscopy of Directed Cell Migration on Partially Etched Electroactive SAM Gold Surfaces
Author(s) -
Lamb Brian M.,
Westcott Nathan P.,
Yousaf Muhammad N.
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200800400
Subject(s) - fluorescence microscope , fluorescence , microscopy , biophysics , cell , chemistry , nanotechnology , materials science , biochemistry , biology , optics , physics
We combine three new straightforward technologies (summarized in the figure) to obtain complete spatial and visual control of directed cell polarity and migration. We also use a chemoselective, electroactive immobilization strategy to pattern ligands onto partially etched gold surfaces, which are compatible with live cell fluorescence microscopy, and demonstrate the utility of this technique for tracking directed cell migration.