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Saturation Transfer Difference (STD) NMR Spectroscopy Characterization of Dual Binding Mode of a Mannose Disaccharide to DC‐SIGN
Author(s) -
Angulo Jesús,
Díaz Irene,
Reina José J.,
Tabarani Georges,
Fieschi Franck,
Rojo Javier,
Nieto Pedro M.
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200800361
Subject(s) - disaccharide , chemistry , nuclear magnetic resonance spectroscopy , saturation (graph theory) , spectroscopy , mannose , stereochemistry , nuclear magnetic resonance , analytical chemistry (journal) , crystallography , biochemistry , organic chemistry , physics , mathematics , combinatorics , quantum mechanics
Concurrent multiple binding modes of a single ligand can be detected and quantified by saturation transfer difference (STD) NMR spectroscopy. Analysis of experimental and predicted STD initial growing rates has allowed us to determine the precise orientation of Man α(1→2)Man in the minor complex with the carbohydrate recognition domain of DC‐SIGN.