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Site‐Selective and Nondestructive Protein Labeling through Azaelectrocyclization‐Induced Cascade Reactions
Author(s) -
Tanaka Katsunori,
Fujii Yohei,
Fukase Koichi
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200800336
Subject(s) - cascade , lysine , chemistry , cleavage (geology) , posttranslational modification , computational biology , computer science , biophysics , biochemistry , biology , chromatography , amino acid , enzyme , paleontology , fracture (geology)
Labeling lysine : A cascade‐type labeling chemistry was developed by making use of rapid 6π‐azaelectrocyclization. The whole process achieves site‐selective and the nondestructive labeling of target proteins under neutral/physiological conditions, without the need for special cleavage operations directed against the affinity ligands.