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Click Chemistry as an Efficient Method for Preparing a Sensitive DNA Probe for Photochemical Ligation
Author(s) -
Ami Takehiro,
Fujimoto Kenzo
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200800316
Subject(s) - chemistry , click chemistry , dna , ligation , combinatorial chemistry , photochemistry , biochemistry , microbiology and biotechnology , biology
Speed of light : We report a new photosensitive probe that takes advantage of the electronic structural changes associated with the formation of triazole rings in Cu I ‐catalyzed Huisgen 1,3‐dipolar cycloadditions between organic azides and terminal alkynes. We have succeeded in performing photoligation on a timescale of seconds. We also report an efficient procedure using photochemical ligation for the preparation of a DNA probe for quick SNP typing.