Premium
Ultrasensitive Detection of DNA by PNA and Nanoparticle‐Enhanced Surface Plasmon Resonance Imaging
Author(s) -
D'Agata Roberta,
Corradini Roberto,
Grasso Giuseppe,
Marchelli Rosangela,
Spoto Giuseppe
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200800310
Subject(s) - peptide nucleic acid , surface plasmon resonance , oligomer , nanoparticle , nucleic acid , dna , nanotechnology , chemistry , materials science , biochemistry , organic chemistry
Femtomolar single‐base mismatch discrimination : A nanoparticle‐enhanced surface plasmon resonance imaging method for ultrasensitive detection of the selective hybridization between a peptide nucleic acid (PNA) oligomer and its complementary single‐stranded DNA target is described. The method allows a 1 f M sensitivity in single‐base mismatch discrimination with only 150 zeptomoles of the target molecules.