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A Novel Label‐Free Biosensor Using an Aptazyme–Suppressor‐tRNA Conjugate and an Amber Mutated Reporter Gene
Author(s) -
Ogawa Atsushi,
Maeda Mizuo
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200800294
Subject(s) - transfer rna , conjugate , suppressor , gene , translation (biology) , reporter gene , computational biology , biology , microbiology and biotechnology , rna , gene expression , genetics , messenger rna , mathematics , mathematical analysis
Freedom of expression : A theophylline‐dependent aptazyme was tethered to the 5′ terminus of the anticodon‐adjusted suppressor tRNA SerU CUA (aptazyme–suppressor‐tRNA conjugates; AST) and the AST was combined with an amber‐mutated luciferase gene in a prokaryotic cell‐free translation system to construct AST biosensors (see scheme).