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Peptide‐Grafted Nanodiamonds: Preparation, Cytotoxicity and Uptake in Cells
Author(s) -
Vial Stéphanie,
Mansuy Christelle,
Sagan Sandrine,
Irinopoulou Theano,
Burlina Fabienne,
Boudou JeanPaul,
Chassaing Gérard,
Lavielle Solange
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200800247
Subject(s) - fluorescence , biomolecule , peptide , fluorescence microscope , confocal microscopy , cytotoxicity , polyelectrolyte , colloid , biophysics , chemistry , confocal , aqueous suspension , nanotechnology , aqueous solution , materials science , in vitro , biochemistry , polymer , organic chemistry , biology , physics , geometry , mathematics , quantum mechanics , microbiology and biotechnology
Nanodiamonds that were prepared by high pressure/high temperature were functionalised with biomolecules for biological applications. Nanodiamonds (NDs, ≤35 nm) that were coated by silanisation or with polyelectrolyte layers were grafted with a fluorescent thiolated peptide via a maleimido function; this led to an aqueous colloidal suspension that was stable for months. These substituted NDs were not cytotoxic for CHO cells. Their capacity to enter mammalian cells, and their localisation inside were ascertained after labelling the nucleus and actin, by examining the cells by confocal, reflected light and fluorescence microscopy.