Premium
The Making of a Scientist II (Nobel Lecture)
Author(s) -
Capecchi Mario R.
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200800239
Subject(s) - concatemer , homologous recombination , dna , plasmid , somatic cell , gene , biology , computational biology , microbiology and biotechnology , chemistry , genetics , genome
Perturbing genes : The incorporation of >100 HSV‐tk plasmid molecules into a single, ordered, concatemer when injected into somatic cells was the first demonstration of homologous recombination between cointroduced DNA molecules in cultured mammalian cells. From this came first the idea that manipulating this efficient machinery would allow any endogenous cellular gene in cultured cells to be mutated in any chosen way, and, eventually, the award of the 2007 Nobel Prize for Physiology or Medicine.