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Two Lysine Residues are Responsible for the Enzymatic Activities of Indole Prenyltransferases from Fungi
Author(s) -
Stec Edyta,
Steffan Nicola,
Kremer Anika,
Zou Huixi,
Zheng Xiaodong,
Li ShuMing
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200800237
Subject(s) - lysine , indole test , mutagenesis , biochemistry , enzyme , aspergillus fumigatus , aspergillus , site directed mutagenesis , biology , chemistry , stereochemistry , amino acid , computational biology , mutation , microbiology and biotechnology , gene , mutant
Basic more important : The importance of two lysine residues (shown in red in the figure) in the catalysis of indole prenyltransferases from fungi was demonstrated by site‐directed mutagenesis with FgaPT2, FtmPT1 and 7‐DMATS from Aspergillus fumigatus. Mutation of a conserved arginine (green) and aspartate (blue) resulted in only a slight decrease in enzymatic activity.