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An Improved Mechanism‐Based Cross‐Linker for Multiplexed Kinase Detection and Inhibition in a Complex Proteome
Author(s) -
Liu Kai,
Kalesh Karunakaran A.,
Bing Ong Li,
Yao Shao Q.
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200800212
Subject(s) - kinome , proteome , linker , kinase , mechanism (biology) , computational biology , chemistry , substrate (aquarium) , computer science , biochemistry , biology , physics , programming language , quantum mechanics , ecology
Trapping the kinome : An improved mechanism‐based cross‐linker for kinase–substrate pairs has been demonstrated in multiplexed detection of kinase activities present in a complex proteome. The method was further extended to screen for inhibitors of multiple kinases in their native environments.