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TokenRNA: A New Type of Sequence‐Specific, Label‐Free Fluorescent Biosensor for Folded RNA Molecules
Author(s) -
Afonin Kirill A.,
Danilov Evgeny O.,
Novikova Irina V.,
Leontis Neocles B.
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200800183
Subject(s) - rna , fluorophore , biosensor , analyte , computational biology , sequence (biology) , molecular beacon , fluorescence , chemistry , consensus sequence , complementary sequences , combinatorial chemistry , nanotechnology , biochemistry , biology , computer science , biophysics , oligonucleotide , base sequence , gene , physics , chromatography , mathematics , materials science , quantum mechanics , statistics
Token RNA : We demonstrate a technique for sensitive, label‐free, real‐time sequence‐specific recognition of prefolded RNA sequences. We use RNA biosensors designed to form an aptameric pocket for the fluorophore upon programmable, paranemic binding to prefolded analyte RNA sequences without the need for denaturation of the secondary structure of the target. We call these constructs “token RNAs”.