Premium
Peptide Backbone Mutagenesis of Putative Gating Hinges in a Potassium Ion Channel
Author(s) -
Nagaoka Yasuo,
Shang Lijun,
Banerjee Arijit,
Bayley Hagan,
Tucker Stephen J.
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200800133
Subject(s) - gating , mutagenesis , potassium channel , chemistry , biophysics , amino acid , ion channel , kcsa potassium channel , peptide , site directed mutagenesis , biochemistry , stereochemistry , mutation , biology , mutant , receptor , gene
Who's the gatekeeper? The mechanism by which K + channels open and close or “gate” is still not fully understood. In this study, we use unnatural amino acid mutagenesis to change the flexibility of the pore‐lining helices of the Kir2.1 potassium channel at two key glycine residues that have been implicated in channel gating.