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Catalyzed Oxidative Corrosion of Porous Silicon Used as an Optical Transducer for Ligand–Receptor Interactions
Author(s) -
Voelcker Nicolas H.,
Alfonso Ignacio,
Ghadiri M. Reza
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200800119
Subject(s) - cyclam , porous silicon , materials science , transducer , silicon , biosensor , ligand (biochemistry) , nanotechnology , porosity , corrosion , biomolecule , optoelectronics , chemistry , metal , metallurgy , receptor , composite material , biochemistry , physics , quantum mechanics
Biosensors fabricated on the nanoscale offer exciting new avenues in the quest for better understanding and characterization of biological systems. Porous silicon is an ideal nanostructured material for the construction of optical transducer matrices because it is easily functionalized with biomolecular probes and displays strong optical interferences. Here, we show that certain transition metal complexes, including nickel(II)cyclam, are able to induce corrosion in porous silicon films rapidly, generating a strong optical interferometric signal that originates from the porous layer. We subsequently exploit this effect to design a transducer for ligand–receptor recognition. With a nickel(II)cyclam derivative as a catalytic label, DNA‐ and avidin‐binding events are detected by time‐lapse interferometric reflectance spectroscopy in a fast, simple, and inexpensive fashion.