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Copper Resistance in E. coli : The Multicopper Oxidase PcoA Catalyzes Oxidation of Copper(I) in Cu I Cu II ‐PcoC
Author(s) -
Djoko Karrera Y.,
Xiao Zhiguang,
Wedd Anthony G.
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200800100
Subject(s) - multicopper oxidase , periplasmic space , copper , chemistry , oxidase test , stereochemistry , biochemistry , enzyme , escherichia coli , organic chemistry , laccase , gene
Copper export : PcoA and PcoC are soluble proteins expressed to the periplasm as part of the copper‐resistance response of E. coli. PcoC binds both Cu I and Cu II to form air‐stable Cu I Cu II ‐PcoC. The blue multicopper oxidase PcoA has been shown to catalyze oxidation of Cu I bound in Cu I Cu II ‐PcoC to less toxic Cu II , which is released into solution. These two proteins may interact with the outer membrane protein PcoB to export excess copper from the periplasm.