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Covalent Fluorescence Labeling of His‐Tagged Proteins on the Surface of Living Cells
Author(s) -
Hintersteiner Martin,
Weidemann Thomas,
Kimmerlin Thierry,
Filiz Nimet,
Buehler Christof,
Auer Manfred
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200800089
Subject(s) - covalent bond , fluorescence , chemistry , reagent , fluorescent protein , computational biology , fluorescent labelling , biochemistry , nanotechnology , combinatorial chemistry , biophysics , biology , green fluorescent protein , gene , organic chemistry , materials science , physics , quantum mechanics
Labeling His‐tagged proteins on living cells. NTA‐based covalent labeling reagents for His‐tagged proteins were designed by combining the oligo‐His directing effect of Ni‐NTA with the irreversible reaction of a photocrosslinker. Using these new probes, a simple five‐minute protocol for labeling His‐tagged proteins on living cells was derived.