Covalent Fluorescence Labeling of His‐Tagged Proteins on the Surface of Living Cells | Zendy

Hintersteiner Martin | Zendy; Weidemann Thomas | Zendy; Kimmerlin Thierry | Zendy; Filiz Nimet | Zendy; Buehler Christof | Zendy; Auer Manfred | Zendy

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Covalent Fluorescence Labeling of His‐Tagged Proteins on the Surface of Living Cells

Author(s) -

Hintersteiner Martin,

Weidemann Thomas,

Kimmerlin Thierry,

Filiz Nimet,

Buehler Christof,

Auer Manfred

Publication year - 2008

Publication title -

chembiochem

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.05

H-Index - 126

eISSN - 1439-7633

pISSN - 1439-4227

DOI - 10.1002/cbic.200800089

Subject(s) - covalent bond , fluorescence , chemistry , reagent , fluorescent protein , computational biology , fluorescent labelling , biochemistry , nanotechnology , combinatorial chemistry , biophysics , biology , green fluorescent protein , gene , organic chemistry , materials science , physics , quantum mechanics

Labeling His‐tagged proteins on living cells. NTA‐based covalent labeling reagents for His‐tagged proteins were designed by combining the oligo‐His directing effect of Ni‐NTA with the irreversible reaction of a photocrosslinker. Using these new probes, a simple five‐minute protocol for labeling His‐tagged proteins on living cells was derived.

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