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Module Extension of a Non‐Ribosomal Peptide Synthetase of the Glycopeptide Antibiotic Balhimycin Produced by Amycolatopsis balhimycina
Author(s) -
Butz Diane,
Schmiederer Timo,
Hadatsch Bianka,
Wohlleben Wolfgang,
Weber Tilmann,
Süssmuth Roderich D.
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200800068
Subject(s) - peptide , metabolite , glycopeptide , ribosomal rna , amino acid , biochemistry , chemistry , biology , stereochemistry , antibiotics , gene
Don't do it by halves : Genetic engineering of non‐ribosomal peptide synthetases (NRPSs) by module (M) exchange (I) and deletion (II) has previously resulted in amino acid exchange and deletion of the synthesized peptide metabolite. Here we report for the first time the insertion (III) of one complete module into an NRPS assembly line, resulting in an elongated peptide metabolite.