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Thermodynamic Analysis of Nylon Nucleic Acids
Author(s) -
Liu Yu,
Wang Risheng,
Ding Liang,
Sha Ruojie,
Lukeman Philip S.,
Canary James W.,
Seeman Nadrian C.
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200800032
Subject(s) - nucleic acid , oligonucleotide , rna , amide , dna , chemistry , nucleic acid thermodynamics , thermal stability , nucleic acid denaturation , chemical stability , stereochemistry , polymer chemistry , organic chemistry , biochemistry , base sequence , gene
The stability and structure of nylon nucleic acid duplexes with complementary DNA and RNA strands was examined. Thermal denaturing studies of a series of oligonucleotides that contained nylon nucleic acids (1–5 amide linkages) revealed that the amide linkage significantly enhanced the binding affinity of nylon nucleic acids towards both complementary DNA (up to 26 °C increase in the thermal transition temperature ( T m ) for five linkages) and RNA (around 15 °C increase in T m for five linkages) compared with nonamide linked precursor strands. For both DNA and RNA complements, increasing derivatization decreased the melting temperatures of uncoupled molecules relative to unmodified strands; by contrast, increasing lengths of coupled copolymer raised T m from less to slightly greater than T m of unmodified strands. Thermodynamic data extracted from melting curves and CD spectra of nylon nucleic acid duplexes were consistent with loss of stability due to incorporation of pendent groups on the 2′‐position of ribose and recovery of stability upon linkage of the side chains.