Premium
Ionic Liquids and Proteases: A Clean Alliance for Semisynthesis
Author(s) -
Wehofsky Nicole,
Wespe Christian,
Cerovsky Vaclav,
Pech Andreas,
Hoess Eva,
Rudolph Rainer,
Bordusa Frank
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200800025
Subject(s) - semisynthesis , chemistry , proteases , ionic liquid , protease , solvent , organic solvent , aqueous solution , aqueous medium , organic chemistry , peptide , ionic bonding , subtilisin , combinatorial chemistry , catalysis , chemical engineering , biochemistry , enzyme , ion , engineering
Herein we present the first report on protease‐catalysed ligation of cleavage‐sensitive peptide and protein fragments in ionic‐liquid‐containing solvent systems. By applying the newly established [MMIM][Me 2 PO 4 ]/buffer mixture as a reaction medium, significant advantages over purely aqueous or conventional organic solvent‐containing media could be identified, including in particular the use of active wild‐type proteases as biocatalysts, the suppression of any competitive proteolytic side reactions, the high turnover rates compared to classical organic solvents and the high stability of chemically labile reactants.