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Rapid and Efficient DNA Strand Cross‐Linking by Click Chemistry
Author(s) -
Kočalka Petr,
ElSagheer Afaf H.,
Brown Tom
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200800006
Subject(s) - click chemistry , dna , chemistry , alkyne , monomer , covalent bond , azide , uracil , combinatorial chemistry , dynamic covalent chemistry , cross link , organic chemistry , molecule , biochemistry , polymer , supramolecular chemistry , catalysis
Click chemistry has been used to covalently cross‐link complementary DNA strands between bases to form very stable duplexes. Several alkyne‐ and azide‐modified uracil monomers were used to evaluate the effect of the linkers on the efficiency of the click reaction. All cross‐linked duplexes had much higher thermal stabilities than non‐cross‐linked ones, with increases in melting temperature of up to 30 °C. In some cases, the conversion was near‐quantitative, and the reaction was complete in 5 min.