Premium
A Sensitive and Selective Near‐Infrared Fluorescent Probe for Mercuric Ions and Its Biological Imaging Applications
Author(s) -
Tang Bo,
Cui Li Juan,
Xu Ke Hua,
Tong Li Li,
Yang Gui Wen,
An Li Guo
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200800001
Subject(s) - fluorescence , fluorophore , metal ions in aqueous solution , photochemistry , chemistry , photoinduced electron transfer , ion , mercury (programming language) , quenching (fluorescence) , metal , selectivity , electron transfer , biochemistry , organic chemistry , optics , physics , computer science , programming language , catalysis
A new mercury(II) near‐infrared region fluorescent probe 3,9‐dithia‐6‐monoazaundecane‐tricarbocyanine has been designed and synthesized. It consists of two functional moieties: the tricarbocyanine performs as the near‐infrared region fluorophore, and the 3,9‐dithia‐6‐monoazaundecane acts as the selected binding site for metal ions. The near‐IR excitation and emission profiles of the probe can minimize cell and tissue damage and avoid native fluorescence from natural cellular species. It exhibits fluorescence increase upon the binding of the Hg 2+ based on the inhibition of the photoinduced electron transfer quenching mechanism. Excellent sensitivity and selectivity for mercuric ions are observed with this probe. The value of the system is demonstrated by its use in monitoring the real‐time uptake of Hg 2+ within HepG2 cells and five day old zebrafish. The synthesis and remarkable properties of it help to extend the development of metal ions fluorescent probes for biological applications.