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Riboswitches for Enhancing Target Gene Expression in Eukaryotes
Author(s) -
Yamauchi Takahiro,
Miyoshi Daisuke,
Kubodera Takafumi,
Ban Mitsuhiro,
Nishimura Akira,
Sugimoto Naoki
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200700782
Subject(s) - riboswitch , thiamine pyrophosphate , gene expression , gene , computational biology , regulation of gene expression , thiamine , biology , pyrophosphate , genetics , microbiology and biotechnology , biochemistry , non coding rna , enzyme , cofactor
Regulating gene expression : The thiamine pyrophosphate‐dependent thiA riboswitch naturally downregulates gene expression upon thiamine pyrophosphate binding in eukaryotes. In the current study, we used this as the basis to engineer an “on riboswitch” that can upregulate gene expression. The riboswitches developed here may be useful as tools for gene regulation.