Premium
Chemoenzymatic Transfer of Fluorescent Non‐natural Amino Acids to the N Terminus of a Protein/Peptide
Author(s) -
Taki Masumi,
Kuroiwa Hiroyuki,
Sisido Masahiko
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200700721
Subject(s) - transfer rna , amino acid , fluorescence , peptide , biochemistry , fluorescent protein , chemistry , n terminus , green fluorescent protein , enzyme , transferase , protein engineering , biology , peptide sequence , gene , rna , physics , quantum mechanics
Non‐natural fluorescence extension . Leucyl/phenylalanyl‐tRNA–protein transferase from E. coli catalyzes the transfer of hydrophobic amino acids from tRNA to the N terminus of proteins. Here, we have expanded this enzymatic reaction to allow attachment of large fluorescent amino acids to peptides and proteins. Furthermore, we minimized the tRNA structure to microhelices or even to pdCpA. Thus, we provide a novel method for N‐terminal specific fluorescence labeling of proteins as shown in the figure.