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Involvement of Two Distinct N‐ Acetylglucosaminyltransferases and a Dual‐Function Deacetylase in Neomycin Biosynthesis
Author(s) -
Yokoyama Kenichi,
Yamamoto Yasuhito,
Kudo Fumitaka,
Eguchi Tadashi
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200700717
Subject(s) - glycosyltransferase , biosynthesis , glycosylation , biochemistry , enzyme , function (biology) , recombinant dna , chemistry , stereochemistry , dual function , biology , computational biology , gene , genetics , computer science , computer graphics (images) , contouring
A new enzyme family : The glycosylation steps in the biosynthesis of neomycin were clarified through recombinant enzyme assays. NeoD catalyzes the first transglucosaminylation, and NeoK catalyzes the second, while NeoL catalyzes both deacetylations. We found that NeoK and its homologous proteins constitute a novel glycosyltransferase family.