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Enzymatic Glycosylation of Peptide Arrays on Gold Surfaces
Author(s) -
Laurent Nicolas,
Voglmeir Josef,
Wright Adam,
Blackburn Jonathan,
Pham Nhan T.,
Wong Stephen C. C.,
Gaskell Simon J.,
Flitsch Sabine L.
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200700692
Subject(s) - glycosylation , peptide , serine , chemistry , threonine , mass spectrometry , enzyme , biochemistry , monolayer , self assembled monolayer , combinatorial chemistry , chromatography
Play it again, SAM . Peptide substrates immobilised on self‐assembled monolayer (SAM)‐coated gold surfaces were efficiently glycosylated with UDP‐ N ‐acetyl‐α‐ D ‐galactosamine:polypeptide N ‐acetylgalactosaminyltransferase 2 (ppGalNAcT2) to form the α‐ D ‐GalNAc threonine/serine linkage. Label‐free analysis of the reactions were performed by using MALDI‐ToF mass spectrometry.