Enzymatic Glycosylation of Peptide Arrays on Gold Surfaces | Zendy

Laurent Nicolas | Zendy; Voglmeir Josef | Zendy; Wright Adam | Zendy; Blackburn Jonathan | Zendy; Pham Nhan T. | Zendy; Wong Stephen C. C. | Zendy; Gaskell Simon J. | Zendy; Flitsch Sabine L. | Zendy

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Enzymatic Glycosylation of Peptide Arrays on Gold Surfaces

Author(s) -

Laurent Nicolas,

Voglmeir Josef,

Wright Adam,

Blackburn Jonathan,

Pham Nhan T.,

Wong Stephen C. C.,

Gaskell Simon J.,

Flitsch Sabine L.

Publication year - 2008

Publication title -

chembiochem

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.05

H-Index - 126

eISSN - 1439-7633

pISSN - 1439-4227

DOI - 10.1002/cbic.200700692

Subject(s) - glycosylation , peptide , serine , chemistry , threonine , mass spectrometry , enzyme , biochemistry , monolayer , self assembled monolayer , combinatorial chemistry , chromatography

Play it again, SAM . Peptide substrates immobilised on self‐assembled monolayer (SAM)‐coated gold surfaces were efficiently glycosylated with UDP‐ N ‐acetyl‐α‐ D ‐galactosamine:polypeptide N ‐acetylgalactosaminyltransferase 2 (ppGalNAcT2) to form the α‐ D ‐GalNAc threonine/serine linkage. Label‐free analysis of the reactions were performed by using MALDI‐ToF mass spectrometry.

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