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Directed Evolution of Galactose Oxidase: Generation of Enantioselective Secondary Alcohol Oxidases
Author(s) -
Escalettes Franck,
Turner Nicholas J.
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200700689
Subject(s) - enantioselective synthesis , directed evolution , galactose oxidase , biochemistry , alcohol oxidase , chemistry , alcohol , galactose , biocatalysis , biology , recombinant dna , gene , catalysis , reaction mechanism , pichia pastoris , mutant
Enantioselective alcohol oxidases : Variants of galactose oxidase have been identified that catalyse the enantioselective oxidation of (±)‐1‐phenylethanol ( ee =99 %) and a range of related substrates, although the wild‐type enzyme shows no activity towards this class. The active‐site R330M mutation is considered important for binding of the aromatic ring of the substrates.