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In Vivo Imaging of a Bacterial Cell Division Protein Using a Protease‐Assisted Small‐Molecule Labeling Approach
Author(s) -
Chattopadhaya Souvik,
Abu Bakar Farhana B.,
Srinivasan Rajavel,
Yao Shao. Q.
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200700647
Subject(s) - ftsz , protease , tobacco etch virus , cell division , bacterial cell structure , cysteine , chemistry , computational biology , biochemistry , small molecule , cysteine protease , cell , microbiology and biotechnology , biology , bacteria , virus , enzyme , genetics , plant virus , potyvirus
Announce on entry : We present a method for the site‐specific labeling of target proteins using a set of cell permeable small‐molecule probes. The tobacco etch virus (TEV) NIa protease, was used to generate target proteins with an N‐terminal cysteine residue, which was subsequently labeled with thioester probe(s) in a site‐specific and covalent manner. Furthermore, we demonstrate the utility of this approach for the study of FtsZ, an important bacterial cell‐division protein (see figure).