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Self‐Cleavable Bioluminogenic Luciferin Phosphates as Alkaline Phosphatase Reporters
Author(s) -
Zhou Wenhui,
Andrews Christine,
Liu Jianquan,
Shultz John W.,
Valley Michael P.,
Cali Jim J.,
Hawkins Erika M.,
Klaubert Dieter H.,
Bulleit Robert F.,
Wood Keith V.
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200700644
Subject(s) - alkaline phosphatase , luciferin , homogeneous , chemistry , phosphate , enzyme , phosphatase , biochemistry , immunoassay , chromatography , luciferase , biology , transfection , physics , antibody , immunology , gene , thermodynamics
After glow . Reactive chemical adaptors were used to stabilize bioluminogenic phosphates for monitoring alkaline phosphatase (AP) activity (see scheme). The 1,6‐elimination based luciferin phosphate exhibited ultrasensitive ability to detect ∼10 −22 mol of AP enzyme in a homogeneous solution, and picograms of protein in an immunoassay.