Premium
Mutant DNA Polymerase for Improved Detection of Single‐Nucleotide Variations in Microarrayed Primer Extension
Author(s) -
Kranaster Ramon,
Ketzer Patrick,
Marx Andreas
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200700609
Subject(s) - pyrococcus furiosus , primer (cosmetics) , dna polymerase , primer extension , polymerase , microbiology and biotechnology , mutant , genetics , biology , nucleotide , dna , chemistry , gene , archaea , organic chemistry
Mutant detects mutation . A mutant DNA polymerase, which was derived from Pyrococcus furiosus DNA polymerase (M2 in figure), was found to have improved single‐nucleotide discrimination properties, and was used for selective microarrayed‐primer extensions. This approach allowed multiplexed SNP detection.