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Enzymatic N‐terminal Addition of Noncanonical Amino Acids to Peptides and Proteins
Author(s) -
Connor Rebecca E.,
Piatkov Konstantin,
Varshavsky Alexander,
Tirrell David A.
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200700605
Subject(s) - azide , amino acid , dihydrofolate reductase , biotin , escherichia coli , biochemistry , enzyme , chemistry , substrate (aquarium) , polyethylene glycol , conjugated system , combinatorial chemistry , biology , gene , organic chemistry , ecology , polymer
Get connected : A chromatographic assay was used to identify ten noncanonical amino acids that can be appended to the N terminus of peptides and proteins by the L,F‐transferase of Escherichia coli. A model protein substrate, E. coli dihydrofolate reductase, was modified with p ‐ethynylphenylalanine and conjugated to azide–biotin and azide–polyethylene glycol–fluorescein probes (see scheme).