Premium
NMR Spectroscopy Reveals Cytochrome c –Poly(ethylene glycol) Interactions
Author(s) -
Crowley Peter B.,
Brett Keith,
Muldoon Jimmy
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200700603
Subject(s) - ethylene glycol , peg ratio , chemistry , cytochrome c , cytochrome , hydrophobic effect , nuclear magnetic resonance spectroscopy , spectroscopy , stereochemistry , polymer chemistry , organic chemistry , biochemistry , enzyme , finance , economics , physics , quantum mechanics , mitochondrion
Hydrophobic PEG : Chemical‐shift‐perturbation mapping reveals an interaction between poly(ethylene glycol) and the hydrophobic surface surrounding the exposed haem edge of cytochrome c. This suggests that PEG might be a poor choice of crowding agent in protein interaction studies. In contrast, cytochrome c can be embedded in agarose gels with little effect on the NMR spectrum.