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Random Walks to Synthetic Riboswitches—A High‐Throughput Selection Based on Cell Motility
Author(s) -
Topp Shana,
Gallivan Justin P.
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200700546
Subject(s) - riboswitch , selection (genetic algorithm) , motility , computational biology , synthetic biology , random walk , rna , computer science , gene , small molecule , throughput , biology , chemistry , microbiology and biotechnology , genetics , non coding rna , artificial intelligence , mathematics , statistics , telecommunications , wireless
Flip the switch . Riboswitches, which are RNA sequences that undergo a ligand‐induced conformational change to alter gene expression, can be used to reprogram how bacteria respond to small molecules. Here we present a new selection method based on cell motility (outlined in the figure) that rapidly identifies synthetic riboswitches and minimizes cost and effort.