Characterization of a New Glycosynthase Cloned by Using Chemical Complementation | Zendy

Tao Haiyan | Zendy; PeraltaYahya Pamela | Zendy; Decatur John | Zendy; Cornish Virginia W. | Zendy

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Characterization of a New Glycosynthase Cloned by Using Chemical Complementation

Author(s) -

Tao Haiyan,

PeraltaYahya Pamela,

Decatur John,

Cornish Virginia W.

Publication year - 2008

Publication title -

chembiochem

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.05

H-Index - 126

eISSN - 1439-7633

pISSN - 1439-4227

DOI - 10.1002/cbic.200700545

Subject(s) - protein fragment complementation assay , glycoside hydrolase , complementation , chemistry , glycosidic bond , trichlorosilane , cloning (programming) , biochemistry , enzyme , mutant , organic chemistry , gene , silicon , computer science , programming language

Screening glycosynthase activity : We report the cloning and characterization of a new glycosynthase Cel5A:E307G from a family 5 glycosynthase by using a chemical complementation LEU2 enrichment assay. This enzyme catalyzes the efficient synthesis of an endo ‐β‐1,3‐glycosidic linkage between lactose‐fluoride donor and p ‐nitrophenyl β‐cellobioside acceptor substrates.

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