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Characterization of a New Glycosynthase Cloned by Using Chemical Complementation
Author(s) -
Tao Haiyan,
PeraltaYahya Pamela,
Decatur John,
Cornish Virginia W.
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200700545
Subject(s) - protein fragment complementation assay , glycoside hydrolase , complementation , chemistry , glycosidic bond , trichlorosilane , cloning (programming) , biochemistry , enzyme , mutant , organic chemistry , gene , silicon , computer science , programming language
Screening glycosynthase activity : We report the cloning and characterization of a new glycosynthase Cel5A:E307G from a family 5 glycosynthase by using a chemical complementation LEU2 enrichment assay. This enzyme catalyzes the efficient synthesis of an endo ‐β‐1,3‐glycosidic linkage between lactose‐fluoride donor and p ‐nitrophenyl β‐cellobioside acceptor substrates.