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Light‐Switching Excimer Beacon Assays For Ribonuclease H Kinetic Study
Author(s) -
Chen Yan,
Yang Chaoyong James,
Wu Yanrong,
Conlon Patrick,
Kim Youngmi,
Lin Hui,
Tan Weihong
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200700542
Subject(s) - molecular beacon , excimer , chemistry , molecular switch , ribonuclease , rnase p , biophysics , combinatorial chemistry , molecule , biochemistry , fluorescence , biology , rna , oligonucleotide , physics , organic chemistry , optics , gene , dna
We have molecularly engineered light‐switching excimer molecular beacons for real‐time and sensitive analysis of RNase H activity with a high signal‐to‐background ratio. This molecular engineering strategy holds great potential for the development of various effective molecular probes for different enzyme‐activity studies.