Premium
In Situ, Digital‐Like, and Reagentless Discrimination of Label‐Free SNPs of 90‐mer Length with Easily Synthesized Electrochemical DNA Probes
Author(s) -
Ikeda Reona,
Akaishi Ayumi,
Chiba Junya,
Inouye Masahiko
Publication year - 2007
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200700522
Subject(s) - in situ , single nucleotide polymorphism , dna , snp , combinatorial chemistry , nucleotide , molecular inversion probe , hybridization probe , electrochemistry , base pair , chemistry , computational biology , microbiology and biotechnology , nanotechnology , materials science , biology , gene , biochemistry , electrode , genotype , organic chemistry
Finding the mismatches. We have developed new electrochemical DNA probes for single nucleotide polymorphism (SNP) detection that are based on hole transport through duplexes. The probes could be easily synthesized, and discriminated single‐base‐mismatched target DNAs from the corresponding fully matched ones. This assay system allows for the in situ detection of label‐free SNPs of 90‐mer length, and it satisfies the requirements for a user‐friendly SNP sensor that is highly compatible with PCR on‐chip technologies.