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An Artificial Aptazyme‐Based Riboswitch and its Cascading System in E. coli
Author(s) -
Ogawa Atsushi,
Maeda Mizuo
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200700478
Subject(s) - ribozyme , riboswitch , ribosomal binding site , ribosome , computational biology , mammalian cpeb3 ribozyme , chemistry , biology , gene , rna , biophysics , biochemistry , non coding rna
Flick of the switch . We used an aptazyme and an anti‐ribosome binding site (RBS) to construct a novel artificial ribozyme‐based riboswitch that functions in E. coli (see figure). This riboswitch worked well at low temperature and activated its own ribozyme and gene expression in the presence of targets even though it is ribozyme based. We also improved the switching efficiency by constructing a cascading system.