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Caged Substrates for Protein Labeling and Immobilization
Author(s) -
Banala Sambashiva,
Arnold Anke,
Johnsson Kai
Publication year - 2008
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200700472
Subject(s) - fusion protein , chemistry , substrate (aquarium) , biophysics , biochemistry , dna , fusion , biology , recombinant dna , ecology , linguistics , philosophy , gene
Spotlight on protein labeling . The labeling of fusion proteins of O 6 ‐alkylguanine‐DNA alkyltransferase (AGT or SNAP‐tag) is controlled with both spatial and temporal resolution by cageing the substrate with the 1‐(2‐nitrophenyl)ethyl group. The substrates can be uncaged simply by illumination. The light‐controlled dimerization and light‐directed immobilization of SNAP‐tag fusion proteins is shown.