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In Vitro Synthesis of New Enniatins: Probing the α‐ D ‐Hydroxy Carboxylic Acid Binding Pocket of the Multienzyme Enniatin Synthetase
Author(s) -
Feifel Sven C.,
Schmiederer Timo,
Hornbogen Till,
Berg Holger,
Süssmuth Roderich D.,
Zocher Rainer
Publication year - 2007
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200700377
Subject(s) - adenylylation , substrate (aquarium) , stereochemistry , chemistry , in vitro , biochemistry , carboxylic acid , biosynthesis , combinatorial chemistry , enzyme , biology , ecology
Groping in pockets . In order to evaluate the substrate specificity of the adenylation domain of the EA module of enniatin synthetase, a set of 30 α‐ D ‐hydroxy acid derivatives with various modifications in the side chain were synthesized and tested. Due to the low substrate specificity of enniatin synthetase a number of new unnatural enniatins could be synthesised (see scheme for example).