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Non‐Colinear Polyketide Biosynthesis in the Aureothin and Neoaureothin Pathways: An Evolutionary Perspective
Author(s) -
Traitcheva Nelly,
JenkeKodama Holger,
He Jing,
Dittmann Elke,
Hertweck Christian
Publication year - 2007
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200700309
Subject(s) - polyketide , polyketide synthase , complementation , streptomyces , biology , phylogenetic tree , computational biology , gene , biosynthesis , gene cluster , function (biology) , genetics , bacteria , phenotype
Abstract Aureothin and neoaureothin (spectinabilin) represent rare nitroaryl‐substituted polyketide metabolites from Streptomyces thioluteus and Streptomyces orinoci , respectively, which only differ in the lengths of the polyene backbones. Cloning and sequencing of the 39 kb neoaureothin ( nor ) biosynthesis gene cluster and its comparison with the aureothin ( aur ) pathway genes revealed that both polyketide synthase (PKS) assembly lines are remarkably similar. In both cases the module architecture breaks with the principle of colinearity, as individual PKS modules are used in an iterative fashion. Parsimony and neighbour‐joining phylogenetic studies provided insights into the evolutionary process that led to the programming of these unusual type I PKS systems and to prediction of which modules act iteratively. The iterative function of the first module in the neoaureothin pathway, NorA, was confirmed by a successful cross‐complementation.