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Quantitative Labeling of Long Plasmid DNA with Nanometer Precision
Author(s) -
Pljevaljčić Goran,
Schmidt Falk,
Scheidig Axel J.,
Lurz Rudi,
Weinhold Elmar
Publication year - 2007
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200700294
Subject(s) - dna , methyltransferase , biotinylation , cofactor , aziridine , biochemistry , plasmid , streptavidin , biotin , biology , chemistry , enzyme , microbiology and biotechnology , methylation , ring (chemistry) , organic chemistry
On target . DNA methyltransferases deliver the methyl group from the natural cofactor S ‐adenosyl‐ L ‐methionine to defined DNA sequences. Engineered aziridine cofactors were used to trick these enzymes into labeling DNA. Sequence specificity was directly visualized by using electron microscopy. The figure shows streptavidin molecules (encircled) complexed to linearized plasmid DNA that was biotinylated at four positions with the DNA methyltransferase M. Taq I and an aziridine cofactor.