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Biochemical Studies of the Thermal Effects on DNA Modifications by the Antitumor Cisplatin and Their Repair
Author(s) -
Halamikova Anna,
Vrana Oldrich,
Kasparkova Jana,
Brabec Viktor
Publication year - 2007
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200700288
Subject(s) - cisplatin , dna repair , dna , chemistry , hyperthermia , dna damage , in vitro , long term potentiation , biophysics , biochemistry , biology , chemotherapy , genetics , paleontology , receptor
Using biochemical methods, we have examined the effect of two factors that might play a role in the mechanism of the biological activity of cisplatin at elevated temperatures (>37°C). We show that increased temperatures result in distinct alterations in the modification of the target DNA by cisplatin, and in the repair of these modifications. Our in vitro results support the view that the enhanced DNA‐cross‐linking efficiency of cisplatin and the lower efficiency of native DNA repair mechanisms at higher temperature play at least a partial role in the potentiation of the antitumor effects of cisplatin under conditions of mild hyperthermia.