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Isolation of DNA Aptamers for CDP‐Ribitol Synthase, and Characterization of Their Inhibitory and Structural Properties
Author(s) -
AllaliHassani Abdellah,
Pereira Mark P.,
Navani Naveen K.,
Brown Eric D.,
Li Yingfu
Publication year - 2007
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200700257
Subject(s) - aptamer , bifunctional , biochemistry , dna , phosphofructokinase 2 , chemistry , enzyme , rubisco , biology , microbiology and biotechnology , catalysis
Two birds with one stone : Four DNA aptamers were isolated for Bcs1, a bifunctional enzyme that catalyzes both the reduction of ribulose 5‐phosphate by using NADPH and cytidylyltransfer by using CTP. The inhibitory effects of these aptamers towards Bcs1 and their secondary structural properties were investigated (see scheme).