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Labeling Tetracysteine‐Tagged Proteins with a SplAsH of Color: A Modular Approach to Bis‐Arsenical Fluorophores
Author(s) -
Bhunia Anjan K.,
Miller Stephen C.
Publication year - 2007
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200700192
Subject(s) - fluorescence , fluorescent protein , moiety , chemistry , nanotechnology , modular design , fluorophore , combinatorial chemistry , biophysics , green fluorescent protein , biochemistry , materials science , computer science , organic chemistry , biology , physics , quantum mechanics , gene , operating system
Everything nicely tied up . We have synthesized a non‐fluorescent bis‐arsenical targeting moiety that can be tethered to a wide variety of different fluorescent payloads. This strategy greatly broadens the scope of dyes that can be used to label tetracysteine‐tagged proteins.