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Prediction of the Candida antarctica Lipase A Protein Structure by Comparative Modeling and Site‐Directed Mutagenesis
Author(s) -
Kasrayan Alex,
Bocola Marco,
Sandström Anders G.,
Lavén Gaston,
Bäckvall JanE.
Publication year - 2007
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200700179
Subject(s) - candida antarctica , lipase , site directed mutagenesis , mutagenesis , chemistry , computational biology , fungal protein , biochemistry , biology , saccharomyces cerevisiae , enzyme , mutation , yeast , gene , mutant
A number of model structures of the CalA suggested by comparative modeling were tested by site‐directed mutagenesis. Enzyme variants were created where amino acids predicted to play key roles for the lipase activity in the different models were replaced by an inert amino acid (alanine). The results from activity measurements of the overproduced and purified mutant enzymes indicate a structure where the active site consists of amino acid residues Ser184, His366, and Asp334 and in which there is no lid. This model can be used for future targeted modifications of the enzyme to obtain new substrate acceptance, better thermostability, and higher enantioselectivity.