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Real‐Time Monitoring of Nucleic Acid Dephosphorylation by Using Molecular Beacons
Author(s) -
Ma Changbei,
Tang Zhiwen,
Wang Kemin,
Tan Weihong,
Yang Xiaohai,
Li Wei,
Li Zhaohui,
Li Huimin,
Lv Xiaoyuan
Publication year - 2007
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200700171
Subject(s) - dephosphorylation , nucleic acid , biochemistry , biology , dna , molecular beacon , dna repair , phosphatase , chemistry , enzyme , oligonucleotide
Peekin' at the beacon : We have developed a novel approach for real‐time monitoring of nucleic acid dephosphorylation catalyzed by T4 polynucleotide kinase (PNK) based on molecular beacons (see scheme). This approach is simple, rapid, highly sensitive, and has excellent specificity for the substrate. It provides a new tool for studying interactions between proteins and nucleic acids.