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Activity‐Based Protein Profiling for Type I Methionine Aminopeptidase by Using Photo‐Affinity Trimodular Probes
Author(s) -
Qiu WenWei,
Xu Jie,
Li JingYa,
Li Jia,
Nan FaJun
Publication year - 2007
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200700148
Subject(s) - methionine , aminopeptidase , chemistry , click chemistry , biochemistry , profiling (computer programming) , combinatorial chemistry , computational biology , leucine , biology , amino acid , computer science , operating system
Three in one . A trimodular probe ( 1 ) with three functional groups (for target recognition, proximal target crosslinking, and distal reporter tag attachment via click chemistry of a photostable azido‐acetyl group) has been designed. We demonstrate its specificity, sensitivity, and potential for general application in activity‐based protein profiling for type I methionine aminopeptidases.