Pyrene Excimer‐Based Dual‐Emission Detection of a Oligoaspartate Tag‐Fused Protein by Using a Zn II –DpaTyr Probe | Zendy

Honda Kei | Zendy; Fujishima Shohei | Zendy; Ojida Akio | Zendy; Hamachi Itaru | Zendy

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Pyrene Excimer‐Based Dual‐Emission Detection of a Oligoaspartate Tag‐Fused Protein by Using a Zn II –DpaTyr Probe

Author(s) -

Honda Kei,

Fujishima Shohei,

Ojida Akio,

Hamachi Itaru

Publication year - 2007

Publication title -

chembiochem

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.05

H-Index - 126

eISSN - 1439-7633

pISSN - 1439-4227

DOI - 10.1002/cbic.200700146

Subject(s) - pyrene , excimer , dual (grammatical number) , fluorescence , combinatorial chemistry , chemistry , naked eye , nanotechnology , computer science , materials science , physics , organic chemistry , optics , art , literature

A dual‐emission sensing system for proteins tethered to a repeated tetra‐aspartate tag (D 4 ‐tag) was successfully developed by using the binding‐induced excimer formation of the pyrene‐appended Zn II –DpaTyr probe. This allows selective detection of the D 4 ‐tag‐fused protein in a simple and convenient manner by the naked eye.

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