Premium
Pyrene Excimer‐Based Dual‐Emission Detection of a Oligoaspartate Tag‐Fused Protein by Using a Zn II –DpaTyr Probe
Author(s) -
Honda Kei,
Fujishima Shohei,
Ojida Akio,
Hamachi Itaru
Publication year - 2007
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200700146
Subject(s) - pyrene , excimer , dual (grammatical number) , fluorescence , combinatorial chemistry , chemistry , naked eye , nanotechnology , computer science , materials science , physics , organic chemistry , optics , art , literature
A dual‐emission sensing system for proteins tethered to a repeated tetra‐aspartate tag (D 4 ‐tag) was successfully developed by using the binding‐induced excimer formation of the pyrene‐appended Zn II –DpaTyr probe. This allows selective detection of the D 4 ‐tag‐fused protein in a simple and convenient manner by the naked eye.