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An RNase H‐Assisted Fluorescent Biosensor for Aptamers
Author(s) -
Ahn DaeRo,
Yang Eun Gyeong
Publication year - 2007
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200700105
Subject(s) - aptamer , rnase p , fluorescence , dna , rnase h , fluorophore , rna , biosensor , chemistry , biophysics , microbiology and biotechnology , biochemistry , biology , gene , physics , quantum mechanics
Recycling program . A signal amplification strategy was established for aptamer‐based molecular recognition of thrombin with concomitant release of a single‐stranded guard‐DNA (g‐DNA). The g‐DNA then bound to F‐RNA‐Q, which contained a fluorophore and quencher. The fluorescence‐quenched RNA was degraded by using RNase H to give a fluorescence signal, and the undamaged g‐DNA was recycled to yield fluorescence amplification.