Premium
An RNase H‐Assisted Fluorescent Biosensor for Aptamers
Author(s) -
Ahn DaeRo,
Yang Eun Gyeong
Publication year - 2007
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200700105
Subject(s) - aptamer , rnase p , fluorescence , dna , rnase h , fluorophore , rna , biosensor , chemistry , biophysics , microbiology and biotechnology , biochemistry , biology , gene , physics , quantum mechanics
Recycling program . A signal amplification strategy was established for aptamer‐based molecular recognition of thrombin with concomitant release of a single‐stranded guard‐DNA (g‐DNA). The g‐DNA then bound to F‐RNA‐Q, which contained a fluorophore and quencher. The fluorescence‐quenched RNA was degraded by using RNase H to give a fluorescence signal, and the undamaged g‐DNA was recycled to yield fluorescence amplification.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom