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Quaternary Ammonium Zinc Phthalocyanine: Inhibiting Telomerase by Stabilizing G quadruplexes and Inducing G‐Quadruplex Structure Transition and Formation
Author(s) -
Ren Lige,
Zhang Anming,
Huang Jing,
Wang Ping,
Weng Xiaocheng,
Zhang Lixia,
Liang Feng,
Tan Zheng,
Zhou Xiang
Publication year - 2007
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200600554
Subject(s) - antiparallel (mathematics) , telomerase , g quadruplex , chemistry , dna , surface plasmon resonance , biophysics , crystallography , biochemistry , materials science , biology , nanotechnology , nanoparticle , physics , quantum mechanics , magnetic field , gene
Water‐soluble, octacationic zinc phthalocyanine (ZnPc) was found to be a very good G‐quadruplex DNA stabilizer by using UV‐melting studies and DNA polymerase stop assays, and a potent telomerase inhibitor by using the telomeric repeat amplification protocol (TRAP) assay. The compound’s DNA‐binding properties were also studied by surface plasmon resonance (SPR). Furthermore, CD experiments demonstrated that ZnPc could induce intramolecular G‐quadruplex structure transition from the antiparallel to parallel form. More importantly, ZnPc was found to induce parallel structure formation in cation‐deficient conditions. The stability of the induced structure was determined with CD melting assays.